Mouse lymphocyte separation solution
Clearly stratified, with no biotoxicity.
One-click copy of information
Instruction manual- Product Description
- Composition
- Precautions
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I. Product Introduction
The product’s main ingredient is Iodixanol, with a molecular weight of 1550. It is a fully chemically inert, non‑cytotoxic iodinated compound that does not bind to any known biologically functional proteins, does not interfere with cell surface membrane proteins, does not inhibit enzymatic activity, and does not disrupt antigen–antibody reactions. Its mechanism relies on the differential cell densities, utilizing the gravitational acceleration generated by centrifugation to achieve cell separation and purification—making it a commonly used reagent for these purposes.
II. Product Information
Specification: 100 mL/bottle
Endotoxin content <0.5 EU/mL
Osmolality: (280 ± 15) mOsmol/kg Scope of application: Peripheral blood
III. Storage Conditions
Store at 4°C–30°C, protected from light; shelf life: 2 years. Transport at room temperature.
IV. Instructions for Use
1. Collect fresh anticoagulated whole blood (EDTA, sodium citrate, or heparin anticoagulants are all suitable). Dilute the whole blood with an equal volume of an isotonic solution (PBS or saline).
2. Add an appropriate volume of separation solution to the centrifuge tube (the total volume should not exceed two-thirds of the tube’s capacity, as exceeding this limit may compromise the separation efficiency). Carefully layer the diluted blood sample on top of the separation solution, ensuring a clear interface between the two phases.
3. At room temperature, centrifuge with a horizontal rotor at 700×g–800×g for 20–30 minutes.
4. After centrifugation, the bottom of the tube contains red blood cells, the middle layer is the separation medium, and the top layer is plasma. Between the plasma layer and the separation medium lies a thin, relatively dense white layer—this is the mononuclear cell layer, which includes lymphocytes and monocytes. Carefully aspirate the white layer into another centrifuge tube.
5. Dilute to a specified volume with an isotonic solution (PBS, saline, or culture medium), and invert to mix thoroughly. At room temperature, centrifuge at 250 × g using a horizontal rotor for 10 minutes, then discard the supernatant. Repeat the washing step 1–2 times.
6. Resuspend the cells in an isotonic solution (PBS, saline, or culture medium) for later use.
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The main ingredient is Iodixanol.
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V. Precautions
1. This product is photosensitive and should be stored at room temperature, protected from light.
2. Please allow the lymphocyte separation solution to equilibrate to room temperature (18°C–30°C) prior to use. Before opening the vial, invert it several times to mix thoroughly. Perform all procedures under aseptic conditions to prevent contamination.
3. To maintain lymphocyte viability, samples should ideally be fresh.
Keywords
GOONIE
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Goonie Biotechnology (Guangzhou) Co., Ltd.
Room 321–332, Building G5, No. 39 Ruihe Road, Huangpu District, Guangzhou
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