Chloroform substitute
Equal‑volume substitution of chloroform; attenuated, low‑risk, and easy to store.
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Instruction manual- Product Description
- Composition
- Precautions
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Product Storage:
Store at room temperature, protected from light; avoid high temperatures and direct contact with open flames. Shelf life: 1 year. Transport at ambient temperature.
Product Overview:
Chloroform is a highly toxic organic solvent with carcinogenic properties and is subject to regulatory control. This product serves as a chloroform substitute, fully replacing chloroform in RNA extraction using the Trizol reagent method, thereby ensuring efficient RNA isolation.
Instructions for Use
This product is used in the same way as chloroform; simply substitute it for chloroform in equal amounts, while keeping all other procedures unchanged.
Example of experimental results
RNA was extracted from 10 mg of mouse liver using Trizol; the extraction performance when this product replaced chloroform is as follows:
Sample A260/A280 A260/A230 Concentration (ng/µL) 10 mg mouse liver 2.014 2.211 932.320 10 mg mouse liver 2.017 2.217 942.251 
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Product Composition
Item number Component Specifications 400-301 Chloroform substitute 100 mL -
Precautions
1. This product poses a fire hazard when exposed to open flames, high temperatures, or oxidizing agents; always keep it away from such hazardous materials during use and storage.
2. This product, like chloroform, is volatile. Use it in a fume hood or under adequate ventilation to avoid inhalation. After use, promptly reseal and store it to prevent reduced reagent efficacy due to evaporation.
3. When using this product, please wear a lab coat and protective equipment such as gloves, a mask, and a face shield.
Keywords
GOONIE
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Goonie Biotechnology (Guangzhou) Co., Ltd.
Room 321–332, Building G5, No. 39 Ruihe Road, Huangpu District, Guangzhou
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