Lentivirus Concentrate (4X)
Recovery rate > 90%
One-click copy of information
Instruction manualPrice:
¥ 700
Item Number:
100-509
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Specification
- 20mL
- 100mL
隐藏域元素占位
- Product Description
- Composition
- Precautions
-
Product Overview:
The lentivirus concentration kit contains a 4× sterile‑treated concentrate that enables simple, rapid concentration of the vast majority of lentiviral particles. This kit is easy to use: no ultracentrifugation or column‑based concentration is required. Simply mix the 4× concentrate with the lentiviral supernatant at the recommended ratio, incubate briefly, and then centrifuge using a standard centrifuge to obtain concentrated lentiviral particles, with a recovery rate exceeding 90% while preserving high infectivity.
Storage conditions
Sterile, stored at 4°C, stable for six months.
Instructions for Use
1. Collect the virus:
Before virus concentration, pipette the cell culture into a 15 mL centrifuge tube within a biosafety cabinet meeting appropriate standards. Centrifuge at 1000 × g for 5 minutes, discard the pellet, and filter the supernatant through a 0.45 μm syringe filter to remove cells and debris.
2. Concentrated virus:
Mix the lentiviral supernatant with the concentrate at a ratio of 3:1 (3 parts supernatant to 1 part concentrate), and incubate at 4°C for 4 to 6 hours or overnight. During the initial phase, gently mix every 30 minutes (do not place the tube on a shaker; avoid continuous shaking). After incubation, centrifuge at 4,000 × g at 4°C for 30 minutes, then carefully discard the supernatant. Resuspend the viral particles in DMEM or PBS at a volume equal to 1/10 to 1/100 of the original volume, and gently pipette up and down to ensure uniformity.
3. Portioning and Storage:
If not used immediately, the concentrated virus can be aliquoted and stored at −80°C. Avoid repeated freeze–thaw cycles, as they can reduce viral titer.
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Product Composition
Item number Specifications 100-509-20
100-509-100
20 mL
100 mL -
Precautions
1. During experimental procedures, please wear a lab coat and gloves, and work within a standard‑compliant biosafety cabinet. Discard waste liquids and pipette tips in designated waste containers containing disinfectant.
2. This product is intended for research use only and shall not be used for diagnostic or therapeutic purposes.
3. Only PVDF or polyethersulfone (PES) membranes may be used for filtering viral supernatants; nitrocellulose (NC) membranes are not permitted.
4. Lentiviruses are cytotoxic to the target cells; if a large number of target cells die after virus addition, the multiplicity of infection (MOI) should be reduced, and the infection duration shortened.
Keywords
GOONIE
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Goonie Biotechnology (Guangzhou) Co., Ltd.
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