Rapid Total RNA Extraction Kit for Cells
8-minute rapid extraction, 0 phenol, 0 chloroform
One-click copy of information
Instruction manual- Product Description
- Composition
- Precautions
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Product Storage: Elution buffer: It is recommended to aliquot and store at room temperature (15–25°C). Other reagents and spin columns should also be stored at room temperature (15–25°C).
Product Overview:
This kit is designed for the rapid isolation of high‑quality total RNA longer than 200 nt from approximately 1×10^6 cells. It employs a proprietary lysis buffer that efficiently disrupts biological samples and inactivates RNases, ensuring the integrity of the isolated RNA. The kit offers simple, fast operation with stable and reliable performance; it does not use toxic reagents such as phenol or chloroform. The resulting RNA exhibits high yield and purity, making it suitable for applications including RT‑PCR and RT‑qPCR.
Operation Demonstration
Product Advantages:
Fast: High-purity RNA extraction in as little as 8 minutes
Non-toxic: Does not use toxic substances such as phenol or chloroform.
Room temperature: The extraction process is carried out at room temperature.
Sample Application Instructions
For standard cell samples, a recommended starting volume is 1×10^5 to 2×10^6 cells; for samples with uncertain outcomes, it is advisable to begin with 1×10^6 cells and adjust based on the extraction results. For cells with low RNA content, such as T cells and B cells, a sample size of 1×10^6 to 3×10^6 cells is recommended. For rare cell types, consider using the RNAflash™ DCA 1‑step RT qPCR (SYBR Green) kit (Cat. No. 500‑103) or the RNAflash™ DCA 2‑step RT qPCR (probe) kit (Cat. No. 500‑106). When running qPCR on cell samples, a single assay can generate Ct values, with a minimum detectable cell count of 10.
Actual performance test:
Electrophoresis results of RNA extracted from 293F cells at a concentration of 5×10^5.
FAQ
Q: Can small RNA fragments, such as miRNAs, be submitted?
A: No.
Q: Can the total RNA extracted be used for sequencing analysis?
A: Yes.
Q: What downstream experiments can the extracted RNA be used for?
A: RNA extraction is a fundamental experiment in molecular biology, aimed at obtaining high-quality total RNA for downstream applications and analyses, such as quantitative real-time PCR, RT‑PCR, Northern blotting, cDNA library construction, and microarray analysis.
Q: How long is the shelf life of the kit?
A: Two years.
Q: Can it be used to extract RNA from small sample volumes?
A: We recommend a sample size of at least 50,000 cells; smaller numbers may result in RNA of lower purity and integrity.
For rare cells, you can choose either the RNAflash™ DCA 1‑step RT qPCR (SYBR Green) kit (Cat. No. 500‑103) or the RNAflash™ DCA 2‑step RT qPCR (probe) kit (Cat. No. 500‑106). Perform qPCR on cell samples to obtain Ct values in a single run, with a minimum of 10 cells supported.
Q: When the extracted RNA is used for sequencing, does the final elution buffer need to be replaced with enzyme‑free water?
A: Check whether the sequencing company has any specific requirements for the final solvent; you can switch to enzyme‑free water.
Q: When the extracted RNA is used for reverse transcription, is genomic DNA (gDNA) removal necessary?
A: Generally, the RNA extracted is free of significant genomic DNA (gDNA) contamination. If contamination does occur, it may be due to an excessive sample volume; in such cases, treat the sample with DNase prior to reverse transcription. (The reverse transcription kit includes a gDNA‑removal enzyme—catalog number 500‑101.)
Product Literature
[1]Tang, M., Xu, Y., & Pan, M. (2025). Integrative Analysis of scRNA-Seq and Bulk RNA-Seq Identifies Plasma Cell Related Genes and Constructs a Prognostic Model for Hepatocellular Carcinoma. Journal of Hepatocellular Carcinoma, 12, 427–444. https://doi.org/10.2147/JHC.S509749
[2]Zheng QY, Xiao LF, An TY, Zhang L, Long X, Wang Q, Wang XZ, Pan HM. IL20RA Is the Key Factor Contributing to the Stronger Antioxidant Capacity of Rongchang Pig Sertoli Cells. Antioxidants (Basel). 2024 Dec 17;13(12):1545. doi: 10.3390/antiox13121545. PMID: 39765872; PMCID: PMC11727484.
[3]Chen B, Liu Y, Luo S, et al. Itaconic acid ameliorates necrotizing enterocolitis through the TFEB-mediated autophagy-lysosomal pathway. Free Radic Biol Med. 2025;226:251-265. doi:10.1016/j.freeradbiomed.2024.11.035
[4]Jiang H, Zhao Y, Jin M, et al. Colchicine Inhibits Smooth Muscle Cell Phenotypic Switch and Aortic Dissection in Mice. Arterioscler Thromb Vasc Biol. Published online April 3, 2025. doi:10.1161/ATVBAHA.124.322252
[5]Liu WH, Chen J, Wu L, et al. Anti-Inflammatory Diterpenoids from the Aerial Parts of Isodon suzhouensis. ACS Omega. 2025;10(16):16658-16667. Published 2025 Apr 18. doi:10.1021/acsomega.5c00223
[6]Chen D, Zhang W, Xiao B, et al. Effect of wild-type vaccine doses on BA.5 hybrid immunity, disease severity, and XBB reinfection risk. J Virol. 2024;98(12):e0128524. doi:10.1128/jvi.01285-24
[7]Hu Y, Xu Y, Gao J, et al. Integrated metabolomics and network pharmacology reveal the mechanisms of Xuebijing in counteracting sepsis-induced myocardial dysfunction. J Ethnopharmacol. 2025;347:119729. doi:10.1016/j.jep.2025.119729
[8]Yang X, Wang B, Zeng H, et al. A Modified Polydopamine Nanoparticle Loaded with Melatonin for Synergistic ROS Scavenging and Anti-Inflammatory Effects in the Treatment of Dry Eye Disease. Adv Healthc Mater. 2025;14(7):e2404372. doi:10.1002/adhm.202404372
Product patent
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Product Composition
Product Number Component (100 times) 400-100A Lysis Buffer 50 ml 400-100B Wash Buffer 15 ml 400-100C Elution Buffer 20 ml 400-100D RNA Purification Column (with Collection Tube) 100 sets Note: Before first use, 45 mL of anhydrous ethanol must be added to the Wash Buffer.
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Precautions
1. The extraction process is carried out at room temperature. Use the recommended sample volume to prevent insoluble precipitates from clogging the spin columns. For adherent cell samples, a confluence of approximately 70% in a 12-well plate or around 40% in a 6-well plate typically yields optimal extraction results. If the cell density in a 6-well plate exceeds 60%, we recommend lysing the cells with 1 mL of lysis buffer directly in the plate, followed by the addition of an equal volume of anhydrous ethanol. Transfer the mixture into two spin columns and proceed according to the instructions provided in the manual.
2. After opening the kit, each use must be performed strictly in accordance with the prescribed procedures to prevent RNase contamination; the eluate should be aliquoted into small portions and stored accordingly.
3. Before first use, add 45 mL of anhydrous ethanol to the Wash Buffer.
4. To ensure complete RNA elution, the volume of the elution buffer should ideally be no less than 20 μl. Repeated elutions can increase RNA yield. The elution buffer must be added to…
Do not add liquid to the side walls of the membrane in the center of the RNA purification column.
5. To ensure extraction quality, it is recommended to use RNase‑free reagents and consumables throughout the entire process, particularly during the RNA elution step.
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