RNAflash™ DCA 1-step RT qPCR (probe) kit
Hands-free RNA, a powerful tool for high-throughput qPCR assays
One-click copy of information
Instruction manualPrice:
¥ 4000
Item Number:
500-104
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Specification
- 50T lysis + 100T qPCR
隐藏域元素占位
- Product Description
- Composition
- Precautions
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RNAflash DCA (Direct Cellular Amplification) One‑Step RT qPCR (Probe) Kit is a rapid, highly efficient, end‑to‑end reagent kit that enables direct conversion from “cells” to “amplification results,” suitable for gene expression analysis in cultures ranging from 10 to 10^5 cells. This product eliminates the cumbersome steps of conventional extraction, allowing genomic DNA removal and RNA isolation directly within cell culture plates. Paired with a one‑step probe‑based RT‑qPCR reagent that delivers high efficiency, robust stability, and exceptional specificity, it can generate gene expression data from cells in as little as 30 minutes. The kit is easy to use, compatible with a wide range of cell types—both adherent and suspension—and accommodates varying cell numbers, enhancing throughput while minimizing sample loss during purification and addressing the poor performance often observed with traditional extraction methods at low cell densities. It is also well suited for high‑throughput analysis of cellular RNA on 96‑ or 384‑well plates.
Product Features:
Universality: Gene expression analysis across multiple cell types, enabling robust detection from as few as 10 to as many as 10⁵ conventional cultured cells or rare cell populations.
Purification-free process: Streamlines the conventional RNA extraction workflow, achieving cell lysis within 5 minutes directly in the culture well and completing genomic DNA (gDNA) digestion in under 7 minutes, thereby minimizing RNA loss associated with purification.
High-throughput compatibility: Meets the demand for parallel processing of large-scale cell samples, significantly reducing both time costs and operational errors in high-throughput assays.
High efficiency: The optimized one-step RT qPCR system is compatible with lysis buffers, enabling detection in as little as 30 minutes.
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GOONIE
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Goonie Biotechnology (Guangzhou) Co., Ltd.
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