One-Step PAGE Gel Rapid Preparation Kit, 10%
The colored top-layer gel allows for convenient sample loading, and the bands remain smooth and easy to image.
One-click copy of information
Instruction manualPrice:
¥ 400
Item Number:
600-102
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Specification
- 125 gel (0.75 mm)
隐藏域元素占位
- Product Description
- Composition
- Precautions
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Product Overview
This kit is designed for the one-step preparation of acrylamide gels, employing a pre-mixed upper and lower gel system. Simply mix the reagents in pairs and add a polymerization accelerator to initiate gelation—no additional TEMED is required, ensuring convenience and efficiency. After pouring the lower gel, no liquid sealing is needed; proceed directly to pour the upper gel. The supplied upper gel comes in four colors (red, yellow, green, and blue), facilitating sample loading and easy differentiation of gels containing different samples, with the dye having no adverse effect on electrophoresis or staining. This kit can prepare 125 mini PAGE gels (calculated based on a 0.75 mm‑thick gel), and the resulting gels are suitable for both denaturing and native PAGE electrophoresis.
Item number and specifications
Item number Amount of glue that can be produced 600-100 (Preparation of 6% PAGE gel) 125 pieces (0.75 mm adhesive)
or
>90 pieces (1.00 mm adhesive)
or
>60 pieces (1.50 mm adhesive)
600-101 (Preparation of 8% PAGE Gel) 600-102 (Preparation of 10% PAGE Gel) 600-103 (Preparation of 12% PAGE Gel) 600-104 (Preparation of 15% PAGE Gel) Gel Preparation Process
(Taking the preparation of a 0.75/1.0/1.5 mm mini gel as an example)
Underlayer adhesive formulation Topcoat formulation Gel thickness Bottom adhesive A1 Lower-layer adhesive A2 Coagulant Gel thickness Top-layer adhesive B1 Top-layer adhesive B2 Coagulant 0.75 mm 2.0 ml 2.0 ml 40 µl 0.75 mm 0.5 ml 0.5 ml 6 µl 1.0 mm 2.7 ml 2.7 ml 60 µl 1.0 mm 0.75 ml 0.75 ml 9 µl 1.5 mm 4.0 ml 4.0 ml 80 µl 1.5 mm 1.0 ml 1.0 ml 12 µl Please follow the steps below precisely. Before use, invert and mix each component 5–10 times.
1. Take equal volumes of lower-layer gel A1 and lower-layer gel A2, 2.0/2.7/4.0 mL each, mix thoroughly, and set aside.
2. Take equal volumes of upper-layer gel B1 and upper-layer gel B2, 0.5/0.75/1.0 mL each, mix thoroughly, and set aside.
3. Add 40/60/80 μl of the modified coagulant to the mixture prepared in Step 1, vortex immediately to mix thoroughly, and then pour the resulting solution into the gel‑casting glass plate, ensuring that the distance between the liquid surface and the top edge of the short glass plate is 0.5 cm longer than the comb teeth.
Note: This solution is in excess; do not inject it all.
4. Add 6/9/12 μl of the modified coagulant to the mixed solution from Step 2, immediately vortex to mix thoroughly, and without waiting for the lower layer to gel, gently inject the mixed solution into the gel‑casting glass plate. Insert the comb, and allow the gel to solidify (at room temperature, approximately 10–20 minutes).
Note: When pouring the upper-layer gel solution, add it gently and slowly to avoid flushing it into the lower layer.
After the gel has solidified, the flatness of the interface between the upper and lower gel layers is slightly inferior to that of gels prepared by the conventional method, but this does not significantly affect subsequent electrophoresis.
5. Electrophoresis voltage: 100–150 V; may be adjusted according to personal preference.
Note: Typically, the upper-layer gel is subjected to electrophoresis at 100–120 V, while the lower-layer gel is run at 120–150 V.
Process Diagram
Reference for Gel Concentration Selection
Separation gel concentration Optimal separation range 6% 50-150 kDa 8% 30-90 kDa 10% 20-80 kDa 12% 12-60 kDa 15% 10-40 kDa Attachment: One-step gel preparation; electrophoresis gel image.
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Product Composition/Storage Conditions
Component Name Volume Save Validity period Transportation Top-layer adhesive B1 80 ml 2-8℃ 12 months Coagulant ice packs are to be transported; other components are to be transported at room temperature.
Top-layer adhesive B2 80 ml 2-8℃ 12 months Bottom adhesive A1 250 ml 2-8℃ 12 months Lower-layer adhesive A2 250 ml 2-8℃ 12 months Improved coagulant
8 ml 2-8℃ 3 months -20℃ 12 months -
Precautions
1. The recommended dosage of the accelerator is for reference only; the actual amount may be adjusted based on room temperature and personal experience. Once opened, the accelerator can be stored at 4°C for at least three months.
2. Gelation rate is significantly influenced by temperature: at excessively high room temperatures, the amount of accelerator may be reduced accordingly; at lower room temperatures, the gelation time can be appropriately extended or the accelerator dosage increased.
3. Before preparing the gel, allow the reagent solution to equilibrate at room temperature for a period of time; this helps promote gelation and effectively prevents bubble formation within the gel.
4. Do not add all of the upper-layer gel; leaving some volume allows you to easily monitor the gel’s setting state.
5. If multiple gels need to be prepared, it is recommended to load no more than the equivalent of two gels per tube at a time. Adding too much reagent may cause partial gelation before pouring into the glass plates, which can compromise gel quality and adversely affect subsequent experiments.
6. Acrylamide is neurotoxic; please wear a lab coat and use disposable gloves and a mask when handling it.
Keywords
GOONIE
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