Serum-free cryopreservation solution
Ready to use—no pre‑cooling required; cell recovery rate > 90%.
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Instruction manual- Product Description
- Composition
- Precautions
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I. Product Introduction
The cell cryopreservation solution is a ready-to-use product that contains no serum or animal-derived proteins. As a result, it not only minimizes the risk of contamination by bacteria, viruses, mycoplasma, and other microorganisms, ensuring the safety of cryopreserved cells, but also reduces the adverse effects of exogenous proteins on normal cell growth and differentiation. It is suitable for a wide range of animal cell lines, including both tumor cells and primary cells.
II. Product Features
1. Serum-free and free of animal-derived components. High safety profile.
2. Ready to use—no freezing required. Stable when stored at 2–8°C. Shipped with ice packs.
3. No programmed cooling is required; directly freeze at −80°C, and after 12 hours transfer to liquid nitrogen.
4. Cell recovery rate > 90%.
III. Operating Steps
(1) Cell Cryopreservation:
1. Collect logarithmically growing adherent or suspension cells into a test tube using standard procedures (Note: Adherent cells must be dissociated into suspension cells).
2. Determine the required number of cells to be cryopreserved based on the cell density and the size of the cryovial.
3. Transfer the required volume of cell suspension into a centrifuge tube, centrifuge at 1,000 × g for 5 minutes to pellet the cultured cells, and carefully discard the supernatant.
4. Add an appropriate volume of cell cryopreservation solution to a centrifuge tube, adjusting the cell concentration to approximately 5 × 10^5 – 1 × 10^7 cells/mL. Gently mix the cells to prepare the cell suspension.
5. Aliquot the cell suspension from the centrifuge tube into labeled cryovials, with 1 mL or 1.5 mL per vial recommended.
6. Place the aliquoted cell cryovials directly into a −80°C ultra-low temperature freezer for long-term cryopreservation.
7. If you wish to store cells in liquid nitrogen for the long term, first place them in a −80°C freezer for at least one day before transferring them to the liquid nitrogen tank. (Note: Use cryovials that are suitable for cryopreservation in liquid nitrogen.)
(2) Cell Revival:
1. Remove the cryovial from a −80°C freezer or liquid nitrogen tank, verify that the cap is tightly sealed, and immediately place it in a 37°C water bath for rapid thawing (to prevent ice crystals from recrystallizing and causing cell death). Gently shake the vial to ensure complete thawing within 1–2 minutes.
2. Once the cell suspension in the cryovial has completely thawed, immediately add 1 mL of cell culture medium to the cryovial and mix thoroughly. Transfer the mixture into a centrifuge tube containing approximately 5 mL of the same cell culture medium, and centrifuge at 1,000 × g for 5 minutes to pellet the frozen cells. Carefully discard the supernatant, taking care not to remove the cell pellet.
3. Add the pre-warmed complete culture medium, mix thoroughly, transfer to a cell culture dish or flask, and incubate in a cell culture incubator.
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IV. Precautions
1. Please ensure that the cryovials are completely sealed to prevent them from bursting during the thawing process.
2. This product contains DMSO; for cells that are sensitive to DMSO, a preliminary experiment is recommended.
3. For certain precious cell types, it is recommended that, prior to the first use of this product, you conduct a trial cryopreservation and culture of the cells for at least one week to verify their performance before proceeding with formal cryopreservation.
4. After aliquoting, cryopreserved cells should be transferred to a −80°C ultra-low temperature freezer as soon as possible to minimize the time they spend at ambient conditions.
5. For your safety and health, please wear a lab coat and disposable gloves while performing this procedure.
6. Observe aseptic techniques to prevent cell contamination.
7. This product is intended for research use only.
Keywords
GOONIE
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Goonie Biotechnology (Guangzhou) Co., Ltd.
Room 321–332, Building G5, No. 39 Ruihe Road, Huangpu District, Guangzhou
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