0.25% trypsin & 0.02% EDTA (with phenol red)
2-minute rapid digestion, high purity and high activity
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Instruction manual- Product Description
- Composition
- Precautions
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I. Product Overview
Trypsin-EDTA Solution contains 0.25% trypsin and 0.02% EDTA (0.53 mM), dissolved in calcium‑ and magnesium‑free HBSS. It is sterilized by two sequential filtrations through a 0.1 µm membrane filter and can be used directly for the digestion of cultured cells and tissues. This product offers convenience, speed, stability, safety, and excellent cell viability. Typically, at room temperature, incubation for about 2 minutes is sufficient to detach most adherent cells.
Trypsin-EDTA digestion solution contains phenol red, which serves as a pH indicator.
II. Storage Conditions
Store at -20°C. Transport with ice packs.
III. Instructions for Use
For adherent cells
1. Aspirate the culture medium, then wash the cells once with sterile PBS, Hanks’ solution, or serum-free culture medium to remove any residual serum.
2. Add a small volume of trypsin–EDTA solution just enough to cover the cells, and incubate at room temperature for 1–2 minutes. Digestion times may vary depending on the cell type; for cells that adhere strongly to the substrate, the digestion time can be extended accordingly.
3. Under microscopic observation, cells exhibit obvious shrinkage, and macroscopic examination of the bottom of the culture vessel reveals marked changes in cell morphology; or
Using a pipette to gently detach the cells, we observed that the cells could be readily detached. At this point, aspirate the digestion solution, add serum-containing culture medium, and gently pipette the cells to dissociate them; the cells can then be directly used in subsequent experiments.
4. If insufficient digestion is observed, add trypsin-EDTA solution and proceed with re‑digestion.
5. If cell digestion is prolonged and the cells are not promptly resuspended by pipetting, some cells may already have detached from the bottom of the culture vessel. In this case, add serum‑containing culture medium and gently resuspend all the cells. Centrifuge at 1,000–2,000 × g for 1 minute to pellet the cells, then carefully remove as much of the trypsin‑based digestate as possible. Finally, resuspend the cells in complete culture medium containing serum; they are now ready for downstream experiments.
For the organization’s digestion
The time required for tissue digestion varies considerably among different organizations; generally, it is advisable to continue digestion until the tissue can be thoroughly dissociated.
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Precautions
1. Due to differences in tissue or cell type, experimental personnel should determine the optimal digestion time based on the specific circumstances; the digestion period should not be excessively prolonged, as this may adversely affect cell attachment and growth.
2. This product does not contain a bacteriostatic agent; during use, strict aseptic techniques must be observed to prevent contamination of the digestion solution by microorganisms.
3. Do not store at 4°C for extended periods; avoid repeated freeze–thaw cycles. For small‑volume use, we recommend aliquoting and cryopreserving.
4. For your safety and health, please wear a lab coat and disposable gloves while performing this procedure.
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Goonie Biotechnology (Guangzhou) Co., Ltd.
Room 321–332, Building G5, No. 39 Ruihe Road, Huangpu District, Guangzhou
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