One-step total cellular RNA extraction kit
5-minute rapid extraction, efficiently isolating total RNA (including small RNAs shorter than 200 bp)
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Instruction manual- Product Description
- Composition
- Precautions
-
Item number: 400-109
Product Storage: All reagent components are at room temperature ( 15-25 Under ℃ conditions, the product should be transported and stored; the shelf life is one year.
Product Overview:
This product is based on silica gel membrane purification technology and, in combination with a unique lysis‑binding solution, allows for one‑step sample loading. Subsequent optimized washing steps effectively remove impurities such as proteins and salt ions, yielding high‑quality total nucleic acids. RNA , the product can be used directly for RT-PCR、qRT-PCR、 Northern Blot 、 Dot Blot , molecular biology experiments such as in vitro translation and high-throughput sequencing.
Product Composition
Product Number
Component
100T
400-109A
CRL buffer
52.5 mL
400-109B
RNA Wash ing Buffer 1
24 mL
400-109C
RNA Wash ing Buffer 2
15 mL
400-109D
RNase-free ddH 2 O
10.5 mL
400-109E
GA2 Adsorption Column (with Collection Tube)
100 sets
Before first use, please first… RNA Washing Buffer 1 and RNA Washing Buffer 2 Add the specified amount. Anhydrous ethanol , After joining, please promptly check the box to indicate that you have added ethanol, to avoid adding it multiple times!
Pre-experiment preparation
According to the instructions in the table below, treat the RNA with [anhydrous ethanol]. Wash ing Buffer 1 and RNA Wash ing Buffer 2. Perform dilution; store at room temperature after dilution.
Component
Amount of anhydrous ethanol added
Wash ing Buffer 1
Product Composition
Product Number
Component
100T
400-109A
CRL buffer
52.5 mL
400-109B
RNA Wash ing Buffer 1
24 mL
400-109C
RNA Wash ing Buffer 2
15mL
400-109D
RNase-free ddH 2 O
10.5 mL
400-109E
GA2 Adsorption Column (with Collection Tube)
100 sets
Before first use, please first… RNA Washing Buffer 1 and RNA Washing Buffer 2 Add the specified amount. Anhydrous ethanol , After joining, please promptly check the box to indicate that you have added ethanol, to avoid adding it multiple times!
Pre-experiment preparation
According to the instructions in the table below, treat the RNA with [anhydrous ethanol]. Wash ing Buffer 1 and RNA Wash ing Buffer 2. Perform dilution; store at room temperature after dilution.
Component
Amount of anhydrous ethanol added
Wash ing Buffer 1
36 mL
Wash ing Buffer 2
45 mL
Precautions
- When using this product, be sure to take appropriate personal protective measures, such as protective clothing, gloves, goggles, and a face shield.
- This kit is intended for research use only and is not to be used for in vitro diagnostic purposes.
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Goonie Biotechnology (Guangzhou) Co., Ltd.
Room 321–332, Building G5, No. 39 Ruihe Road, Huangpu District, Guangzhou
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