GelRed Nucleic Acid Stain 10,000×
High sensitivity; performance can fully replace EB.
One-click copy of information
Instruction manual- Product Description
- Composition
- Precautions
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Product Introduction
GelRed is a next-generation nucleic acid dye that can replace EB. It exhibits spectral properties identical to those of EB, emitting red fluorescence under UV excitation; the intensity of the fluorescent signal increases with the amount of nucleic acid present. Moreover, GelRed is safer, more stable, and more sensitive. It is widely used for staining RNA or DNA in gels. Its large molecular structure cannot penetrate cell membranes, making it non‑toxic and non‑mutagenic—thus safer than EB.
Storage conditions
Store away from light at room temperature (15°C–25°C); shelf life is two years. Transport at ambient temperature.
Instructions for Use
1. Pre-staining method: Prepare the gel using GelRed:
1) Weigh the agarose according to the experimental requirements, add TAE buffer, and heat it in a microwave until fully melted.
2) Add GelRed to the gel solution at a ratio of 1:10,000 (e.g., add 1 μL of this product to 10 mL of gel), and mix thoroughly.
3) Pour the well-mixed liquid into the gel‑casting tray, insert an appropriate comb, and allow it to cool and solidify.
4) Remove the solidified agarose gel and proceed with sample loading and electrophoresis.
5) After electrophoresis is complete, remove the gel from the electrophoresis tank and examine it under UV light or take a photograph.
2. Post-staining method: Staining the gel after electrophoresis:
1) Prepare the gel‑staining solution by adding GelRed to water or to a 100 mM NaCl solution at a ratio of 1:2,500–5,000 (e.g., add 20–40 μL of this product to 100 mL of water or NaCl solution), and mix thoroughly; set aside. The staining solution can be reused up to three times. Store the prepared solution at room temperature, protected from light.
2) After preparing the gel according to standard procedures and completing nucleic acid electrophoresis, transfer the gel to an appropriate container and add staining solution, ensuring that the gel is fully immersed.
3) Place the gel immersed in the staining solution on a shaker for staining. Stain for approximately 30 minutes; the duration can be adjusted according to the gel thickness—extend the staining time for thicker gels and shorten it for thinner ones. Polyacrylamide gels can be stained for 30–60 minutes; the higher the polyacrylamide concentration, the longer the staining time should be.
4) (Optional) The stained gel can be rinsed 1–2 times with deionized water to reduce background signal.
5) Observe or photograph under ultraviolet light.
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Constituent components
Item number Name Specifications 400-110 GelRed nucleic acid dye 500 μL -
Precautions
1. During the experiment, please wear a mask and gloves.
2. This product is intended for research use only; do not use it for in vitro diagnostic or other therapeutic purposes.
3. For polyacrylamide gels, please use this product according to the post-staining method.
4. When using the pre‑staining method, nucleic acid dyes may interfere with nucleic acid migration; if this affects result interpretation, switch to a post‑staining method.
5. Do not reuse gels that have already been used for electrophoresis.
Keywords
GOONIE
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Goonie Biotechnology (Guangzhou) Co., Ltd.
Room 321–332, Building G5, No. 39 Ruihe Road, Huangpu District, Guangzhou
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