Anti-GAPDH recombinant mAb [ MGP-01]
Application:
WB, IP, IHC-P, ICC/IF, FC (Intra)
Species reactivity:
Human, Mouse, Rat, Pig, Zebrafish, Yeast, Monkey
Host species:
Mouse
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Instruction manual- Product Description
- Composition
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Product Overview:
Product name: anti-GAPDH recombinant mAb [MGP-01]
Antibody type: Monoclonal
Validated applications: WB, IP, IHC-P, ICC/IF, FC (Intra)
Host: Mouse
Species reactivity: Human, Mouse, Rat, Pig, Zebrafish, Yeast, Monkey
Clone number: MGP-01
Isotype: Mouse IgG2b.Background of the target:
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a highly conserved enzyme involved in the glycolytic pathway. It catalyzes the conversion of D-glyceraldehyde 3-phosphate to 3-phospho-D-glyceroyl phosphate. GAPDH is more than just a glycolytic enzyme; it also participates in processes such as cell signaling, programmed cell death, and DNA repair.
Therefore, GAPDH is considered to be associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
GAPDH is commonly regarded as a housekeeping gene due to its critical role in energy metabolism and its widespread
expression across various tissues and cell types. As such, it is often used as a loading control in Western blotting and RT-PCR. Its consistent expression across different experimental conditions makes it a reliable reference for normalization in
quantitative analysis.
SwissProt: P04406
Alternate names: Glyceraldehyde-3-phosphate dehydrogenase, GAPD
Application Dilutions
Western blot
1:10000 - 1:50000
IHC-P
1:3000 - 1:16000
ICC/IF
1:400 - 1:1600
Flow cytometry
Use 0.4 µg of antibody per 10^6 cells in a 100 µL staining volume.
IP
Use 0.5 - 4.0 µg of antibody per 2 - 3 mg of total protein lysate.
Product images
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Storage: Store at 2–8°C, protected from light. Transport with ice packs.
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