PE Streptavidin
Application:
Species reactivity:
Host species:
One-click copy of information
Instruction manual- Product Description
- Composition
- Precautions
-
Product item number: NF0002-5 (100μg) NF0002-6 (500μg)
Experimental application: Flow Cyt, IF
Product Information
Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.
Other Names: Streptavidin-Phycoerythrin, SAv-PE
Excitation Laser: Blue Laser (488 nm)/ Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Composition
Item number/specification
Component Name
Volume
NF0002-5 (100μg)
PE Streptavidin (0.2 mg/mL)
0.5 mL
NF0002-6 (500 μg)
PE Streptavidin (0.2 mg/mL)
2.5 mL
Storage: Store at 2–8°C, protected from light. Transport with ice packs. Do not freeze.
Instructions for Use
Flow cytometric antibody staining of cell surfaces
1. Resuspend the prepared cells (5–10 × 10^5 cells/tube) in 100 μL of cell staining buffer.
2. Add 0.25–0.5 μg of biotin‑labeled antibody to each tube, gently mix, and incubate in the dark on ice for 15–20 minutes.
3. Wash once with 1 mL of cell staining buffer, then resuspend the cells in 100 µL of cell staining buffer.
4. Add 1 μL (0.5 μg) of PE‑streptavidin to each tube and incubate in the dark on ice for 20 minutes.
5. Wash once with 1 mL of cell staining buffer.
6. Resuspend the cells in 300–500 μL of cell staining buffer and perform the assay promptly. If immediate analysis is not possible, store protected from light at 4°C and complete the assay within 4 hours.
Precautions
Before use, briefly centrifuge to recover droplets adhering to the tube walls.
During use, avoid exposure to light.
This product is intended for research use only and is not to be used for in vitro diagnostic purposes.
Product test data:
Human peripheral blood lymphocytes stained with C
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Product Composition
Item number/specification
Component Name
Volume
NF0002-5 (100μg)
PE Streptavidin (0.2 mg/mL)
0.5mL
NF0002-6 (500 μg)
PE Streptavidin (0.2 mg/mL)
2.5mL
Storage: Store at 2–8°C, protected from light. Transport with ice packs. Do not freeze.
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