Product Overview

TL;DR ： FiniGet Fenbide® 120-6005 Human NK Cell Isolation Kit, utilizing Biotin–streptavidin immunomagnetic bead assay ， 15 min rapid sorting; purity can be increased from 15.9% before sorting to 87.6% after sorting. (Human PBMC, manufacturer’s testing); No red‑splitting treatment is required before use; it can be applied directly by pouring. , sorting cell surface No antibody/magnetic bead labeling, no abnormal activation. ， Compatible with human peripheral blood mononuclear cells (PBMCs) ， Enrich NK cells , widely used in Research on NK cell function, tumor immunotherapy, viral infection immunity, transplant immunity, and autoimmune diseases. For research experiments, store at 2–8°C; shelf life is one year.

Core Parameters Summary Table

Product Features

Fast and efficient : Negative selection using immunomagnetic beads, 15 min completed (Cocktail 10 min + Beads 2.5 min + Magnetic separation 2.5 min), with no centrifugation required throughout the entire process.

High purity, high activity : Purity can be increased from 15.9% prior to sorting to 87.6% post-sorting (human PBMCs, manufacturer‑tested), with no abnormal activation of the sorted cells.

Easy to operate : No red‑splitting treatment is required; the pour‑off method can be used directly. No separation column is needed, saving both experimental costs and time.

No surface markings : Negative selection does not involve contact with the target cell surface; NK cells do not bind to any antibodies or magnetic beads, thereby maintaining their original quiescent state.

Fully equipped : To be used with the FiniGet Separation‑by‑Dilution® 140‑2000 single‑well magnetic rack and the 140‑2001 cell‑sorting buffer; ready to use straight out of the box.

Operation Flowchart

Flowchart of the Human NK Cell Isolation Kit, FiniGet 120-6005, Negative Selection Protocol

Instructions for Use

This procedure is suitable for processing samples ranging from 0.1 to 2 mL (up to 2×10⁸ cells), using FiniGet Fenbide® 140–2000 single-hole magnetic stands and 5 mL sterile flow cytometry tubes.

Taking the sorted human PBMC NK cells as an example:

Operating Procedure (Starting with PBMCs, Pouring Method) ：

1. Preparation of human PBMCs : Utilize Ficoll density gradient centrifugation PBMCs were isolated from human peripheral blood. The PBMCs were collected, washed with PBS, and after centrifugation, resuspended in sorting buffer at a cell density of… 1×10⁸ cells/mL 。

· If cryopreserved PBMCs are used, the cells will form clumps after thawing. Prior to sorting, the sample should be incubated at room temperature in a 100 μg/mL DNase I solution for at least 15 minutes and then filtered through a cell strainer.

· For the sorting buffer, we recommend using PBS containing 1 mM EDTA and 2% fetal bovine serum (FBS), which should be filtered through a 0.22 μm membrane filter to remove bacteria prior to use. The buffer must not contain calcium ions, magnesium ions, or biotin. Alternatively, you may also use FiniGet Fenbide® 140-2001 Cell Sorting Buffer.

2. Pipette 100 μL of cell suspension (1×10⁷ cells) into the bottom of the flow cytometry tube, then add 10 μL Human NK Cell Isolation Cocktail , mix well, Incubate at room temperature for 10 min. 。

· If sorting a larger number of cells, the amount of Human NK Cell Isolation Cocktail can be increased proportionally.

3. Add 10 μL Streptavidin Beads (Shake vigorously for about 30 seconds before use; do not centrifuge at high speed after mixing.) Mix well, Incubate at room temperature for 2.5 min. 。

· If sorting a larger number of cells, the amount of Streptavidin Beads can be increased proportionally.

4. Add sorting buffer to bring the volume to 2.5 mL , after gentle mixing, place the flow cytometry tube into the magnetic stand. Let stand for 2.5 min 。

· The experimental volume should not exceed 2.5 mL to prevent exceeding the magnetic separator’s sorting range.

5. Pick up the magnetic holder, Pour gently and smoothly. The cell suspension is placed in a sterile centrifuge tube, with the magnetic poles and flow cytometry tubes kept in an inverted position. 2-3 s Subsequently, it returns to an upright orientation. This suspension contains purified human NK cells.

· During the pouring process, the flow cell must remain attached to the magnetic stand.

· Do not shake the tube or touch, via the tube opening, any droplets that may still be clinging to the flow‑cell inlet. Such droplets must be discarded.

6. After washing the cells, resuspend them in the desired buffer or culture medium, and they are ready for downstream applications.

Sorting efficiency

Image Alt : Comparison of flow cytometry results before and after sorting NK cells from human PBMCs—purity increased from 15.9% prior to sorting to 87.6% post-sorting.

NK cells were sorted from human PBMCs, and the cells before and after sorting were used with… PE anti-human CD3 antibody (clone OKT3) and APC anti-human CD56 antibody (clone 5.1H11) After labeling, perform flow cytometry analysis. The NK cell purity before and after sorting was 15.9% and 87.6%, respectively. 。

Customer Feedback ：

Customer Feedback from Sun Yat-sen University Cancer Center (Human NK negative selection): Purchased Cryopreserved cord blood mononuclear cells (CBMC) (There are red blood cells before sorting), initial 0.9×10⁷ cells (viability 90%) , must obtain 120–6005 points after sorting 1.3×10⁶ cells (viability 90%) ， Purity 92.1% 。

Application scenarios

Research on NK Cell Function : NK cell activity assay (cytotoxicity assay), analysis of NK cell receptor expression (NKG2A, NKG2D, KIR), and cytokine secretion profiling (IFN-γ, TNF-α) studies

Tumor Immunotherapy Research : Adoptive cell therapy with NK cells (CAR-NK), research on tumor-infiltrating NK cells (TINK), studies on immune checkpoints (PD-1/NKG2A), and evaluation of cancer vaccines

Research on Immunity to Viral Infections : Research on NK cell responses to viral infections such as CMV, HIV, HCV, and SARS-CoV-2, and studies of the antiviral mechanisms of NK cells

Transplant Immunology Research : Research on NK cell reconstitution following hematopoietic stem cell transplantation, studies on KIR–MHC mismatched transplantation (KIR ligand mismatch), and research on graft-versus-host disease (GVHD)

Autoimmune Disease Research : Research on the mechanisms of NK cell function in autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and multiple sclerosis (MS)

Selection Guide

Recommended for use : From people Peripheral blood mononuclear cells (PBMCs) Obtain quickly in the middle NK cells , used in downstream experiments on NK cell function, tumor immunity, antiviral immune responses, transplant immunity, autoimmune diseases, and more.

Not recommended for use. ：

CD3+ T cells are required. : Please select 120-6001 (Human CD3+ T Cell Sorting Kit)

CD4+ T cells are required. : Please select 120-6002 (Human CD4+ T Cell Sorting Kit)

CD8+ T cells are required. : Please select 120-6003 (Human CD8+ T Cell Sorting Kit)

B cells are required. : Please select 120-6004 (Human B Cell Sorting Kit)

The sample is mouse spleen/lymph nodes. : Please select 130-6005 (Mouse NK Cell Sorting Kit)

The starting sample is human whole blood. : PBMCs must first be isolated by Ficoll density gradient centrifugation, followed by cell sorting.

Frequently Asked Questions (FAQ)

Q: What are the key differences between 120-6005 and 120-6001 / 120-6002 / 120-6003 / 120-6004 / 130-6005?

A: 120-6005 Sorting NK cells , 120-6001 Sorting CD3+ T cells , 120-6002 Sorting CD4+ T cells , 120-6003 Sorting CD8+ T cells , 120-6004 Sorting B cells , 130-6005 Sorting Mouse NK cells , all using the negative selection method with immunomagnetic beads. 120-6005 The starting sample is Human PBMC , 130-6005 The initial sample is Single-cell suspensions of mouse spleen and lymph nodes 。 

 

Q: Is 120-6005 a positive or negative selection?

A: Negative selection. In the kit, Biotin-labeled monoclonal antibody cocktail In combination with human PBMCs Non-NK cells (including T cells, B cells, monocytes, granulocytes, etc.), through Streptavidin magnetic beads Remove these cells, Objective: NK cells do not bind any antibodies on their surface. , keep it in its original state.

Q: Can sorted NK cells (120-6005) be used in activation/culture experiments?

A: Yes. Negative selection. Does not trigger surface receptor cross-linking. , after sorting, NK cells remain in a quiescent state. If activation is required, in conjunction with IL-2 / IL-12 / IL-15 Cultivation and use. 

 

Q: What auxiliary equipment is required for 120-6005?

A: Recommended with FiniGet Fenbide® For use with the magnetic holder: 140-2000 Single-hole magnetic stand (0.1–2 mL / 2×10⁸ cells) . Recommended sorting buffer solution FiniGet Fenbide® 140-2001 Cell sorting buffer . The procedure uses a 70 μm cell strainer and a 50 mL centrifuge tube. 

 

Q: How long does it take to sort NK cells using the 120-6005?

A: 15 min. Cocktail incubation at room temperature for 10 min + Beads incubation at room temperature for 2.5 min + Magnetic bead incubation for 2.5 min. 

 

Q: Which organizations are supported by the 120-6005 initial sample? Is red blood cell lysis required?

A: The initial sample is Human peripheral blood mononuclear cells (PBMCs) , through Ficoll density gradient centrifugation Isolate PBMCs, No red-splitting treatment is required before use. — The PBMC layer contains no red blood cells. After thawing, the cryopreserved PBMCs must be washed with… 100 μg/mL DNase I solution Incubate at room temperature for at least 15 minutes, then filter through a cell strainer to remove cell aggregates. 

 

Q: How does FiniGet compare to cell sorting kits from leading imported brands?

A: Both can achieve high-purity, highly active cell sorting.

The main differences are reflected in five dimensions:

Sorting speed — FiniGet: approximately 15 minutes; top imported brands: approximately 25 minutes.

Separation column —FiniGet eliminates the need for a separation column, whereas imported top-tier brands require the separate purchase of such columns.

Crack Red Steps —FiniGet requires no red‑splitting treatment, whereas imported top‑brand products do.

Operating temperature —FiniGet operates at room temperature, whereas imported top‑brand products require low‑temperature storage at 4°C.

Kit price —FiniGet is approximately one-third to one-half the price of imported brands.

Q: Under what circumstances can you request FiniGet Fenbide® on-site technical support?

A: Applications are welcome for first-time use of the FiniGet® kit, special sample types (rare samples or highly challenging sorting), and group‑wide bulk purchases (3 or more kits). For other needs, please contact customer service for details. In Guangzhou and Shenzhen, we provide a response within 24–48 hours; for other cities, please book at least two weeks in advance. To make an appointment, call 400‑090‑1923 or email service@gooniebio.com. 

 

Q: Is FiniGet’s “FiniGet® On‑Site Technical Support” free of charge? Are there any limits on the number of support sessions?

A: Completely free, with no limits on the number of institutions or sessions. To book, please call 400-090-1923 or email service@gooniebio.com.

Technical Support

FiniGet Fenbide® Affiliated with Albatross Biotechnology (Guangzhou) Co., Ltd., we specialize in immunomagnetic bead–based cell sorting technology. Customer service: 400-090-1923 / service@gooniebio.com

On-site technical support

Having trouble getting your cell-sorting experiment to run successfully for the first time? The FiniGet® technical team can come to your lab and walk you through the entire process—featuring on-site hands-on demonstrations, instrument setup, and comprehensive technical training. Our customer service hotline and technical support team will coordinate with you; after you make an appointment, a dedicated representative will handle all the details. 
 

Coverage and Response Time:

Note: The response times listed above apply to standard circumstances and will be extended during holidays; the exact on-site appointment time is subject to confirmation by customer service.

Page Summary

FiniGet Fenbide® 120-6005 Human NK Cell Isolation Kit, Immunomagnetic Bead-Based Negative Selection, 15 min rapid sorting; purity can be increased from 15.9% before sorting to 87.6% after sorting. (Human PBMC, manufacturer’s testing); No red‑splitting treatment is required before use; it can be applied directly by pouring. , sorted cells No antibody/magnetic bead labeling, no abnormal activation. , compatible Human peripheral blood mononuclear cells (PBMCs) Store at 2–8°C.